Specimens submitted for histopathology must be in 10% neutral-buffered formalin.
The ratio of formalin:tissue is preferably 10:1 with a minimum of 5:1.
Collect samples in wide-opening containers — not tubes and narrow-opening containers. Tissue hardens with fixation, and removal of hardened fixed tissue distorts the tissue histology and is a laboratory hazard.
Specimen containers of 180-250 ml are recommended for immune and digestive tissue surveys, and general diagnostics. For larger specimens like bone, or greater specimen numbers use 380-500 ml containers.
Links to vendor of pre-filled formalin containers.
- https://www.medline.com/product/Prefilled-Formalin-Specimen-Containers/Z05-PF50630
- https://www.thomassci.com/Chemicals/Histology/_/Prefilled-10-Neutral-Buffered-Formalin-Containers?q=Formalin%20Containers
Optional: After fixation (24-48 hours), most formalin can be removed prior to shipping, but leave 5-10 ml to keep the tissue wet. Samples can also be shipped with all of the formalin. Note: dispose of formalin properly – not into the sanitary waste stream. Used formalin can be saved and used when additional formalin is needed for specimens. Contact us for additional information.
Required: Fixation for 3 days (72 hours) is required by US Custom for entry of specimens into the USA.
Specimen Collection Guides
Chicken testicle collection for virology and histopathology
